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KMID : 0613820140240060612
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Journal of Life Science
2014 Volume.24 No. 6 p.612 ~ p.617
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RAPD Variation and Phenetic Relationships for Six Populations of Equisetum pratense in Korea
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Huh Man-Kyu
Choi Jae-Won
Lee Jang-Seop
Jin Bo-Gye
Kim Hyun-Kyung
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Abstract
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The phenetic relationships among six natural populations of Equisetum pratense in Korea were investigated at the population level by constructing a tree based on Random Amplified Polymorphic DNA (RAPD) markers. RAPD analysis was also conducted to estimate genetic diversity and the population structure of E. pratense. A mean of 26.7% at the six population levels indicated polymorphism. E. pratense was found to have fewer alleles per locus (1.267) and fewer effective alleles per locus (1.176). Genetic diversity (0.102) in E. pratense is lower than the average for species with similar life history traits. Total genetic diversity values (HT) varied between 0.112 (OPD-07) and 0.445 (OPD-16), for an average overall polymorphic locus of 0.141. Inter-locus variation in the within-population genetic diversity (HS) was low (0.102). Asexual reproduction, small population size, and the colonization process are proposed as possible factors contributing to the observed low genetic diversity in E. pratense. On a per-locus basis, the proportion of total genetic variation due to differences among populations (GST) ranged from 0.129 for OPD-07 to 0.455 for OPD-09, with a mean of 0.277. This indicated that about 27.7% of the total variation was among populations. Thus, genetic variation (72.3%) resided within populations. This study contributes new information for research on the taxonomy and population genetics of E. pratense.
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KEYWORD
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Equisetum pretense, genetic diversity, genetic variation, population structure, random amplified polymorphic DNA (RAPD)
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